Detection Of Canine Parvo Infection At Different Pet Clinics In Lahore Through Haemagglutination (Ha)
By: Asif Ali
| Prof.Dr.M.Younus Rana.
Contributor(s): Dr.Asim Aslam | Prof.Dr.Kamran Ashraf.
Material type: 
BookPublisher: 2011Subject(s): Department of PathologyDDC classification: 1260,T Dissertation note: Canine parvovirus, caused by a haemagglutinating canine parvovirus (CPV), one of the most important acute viral infectious diseases of pups, had been prevalent in the country. In the present study, 200 faecal and blood samples, from clinically suspected cases of parvovirus diseases dogs were collected from five pet centers of Lahore. Serum samples were harvested for hemaglutination inhibition test while the faecal samples were diluted and centrifuged to collect the supernatant. Being a haemagglutinating (HA) virus, the pre-filtered supernatant from all suspected samples was checked for any HA activity using 1% washed chicken erythrocytes. Out of total of 200 samples, 127 samples were found HA positive. Postmortem of dead dogs suspected for CPV infection was done and various gross pathological lesions were noted. Blood filled intestine lumen was found during postmortem. When lumen of intestine was opened it gave washed out appearance of intestine as mucosa was severely sloughed off. The heart of young puppies showed marbled appearance due to the presence of whitish layer on heart. Tissue samples duodenum, jejunum, ileum and heart were preserved in 10% formalin solution. The CPV cases were also observed in the locally found cross breed. This could be due to the emergence of the new stains of parvo virus due to which the cross breed getting affected.
| Item type | Current location | Collection | Call number | Status | Date due | Barcode | Item holds |
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Thesis
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UVAS Library Thesis Section | Veterinary Science | 1260,T (Browse shelf) | Available | 1260,T |
Canine parvovirus, caused by a haemagglutinating canine parvovirus (CPV), one of the most important acute viral infectious diseases of pups, had been prevalent in the country. In the present study, 200 faecal and blood samples, from clinically suspected cases of parvovirus diseases dogs were collected from five pet centers of Lahore. Serum samples were harvested for hemaglutination inhibition test while the faecal samples were diluted and centrifuged to collect the supernatant. Being a haemagglutinating (HA) virus, the pre-filtered supernatant from all suspected samples was checked for any HA activity using 1% washed chicken erythrocytes. Out of total of 200 samples, 127 samples were found HA positive. Postmortem of dead dogs suspected for CPV infection was done and various gross pathological lesions were noted. Blood filled intestine lumen was found during postmortem. When lumen of intestine was opened it gave washed out appearance of intestine as mucosa was severely sloughed off. The heart of young puppies showed marbled appearance due to the presence of whitish layer on heart. Tissue samples duodenum, jejunum, ileum and heart were preserved in 10% formalin solution. The CPV cases were also observed in the locally found cross breed. This could be due to the emergence of the new stains of parvo virus due to which the cross breed getting affected.
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